In vitro overlay for gametes and zygotes

Some procedures for IVF and ICSI use an oil overlay to protect small volumes of culture medium from evaporation in the incubator and to help dampen the effects of sudden changes in temperature, gas saturation, and pH. Likewise, gamete manipulation under the microscope requires the gametes to be held in small drops of medium under oil, to maintain a constant environment within the culture medium.

Hyaluronic Acid based natural alternative to polyvinyl plastic (PVP) for slowing sperm for ICSI.

SpermCatch is a natural alternative to polyvinylpirrolidone (polyvinyl plastic – PVP) for slowing sperm for ICSI. Clinicians have always been concerned that injecting this artificial product (PVP) into the oocyte along with the sperm may cause damage to acrosomal, mitochondrial and plasma membranes, as well as to the chromosomal DNA.